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In order to obtain mutagenic data of enzymatic browning reaction products, we investigated their mutagenicity. The rec-assay with Bacillus subtilis strains H17(rec^+) and M45(rec^-) were carried out with their spores. Detection of double strand breakage in calf-thymus DNA was investigated into sample solution with and without Cu^(2+) by the agarose slab gel electrophoresis. In the rec-assay, catechol, pyrocatechol, DL-dopa, 3,4-dihydroxytoluene, hydroquinone, hydroxyhydroquinone with and without Cu^(2+) in 0.05M and 0.1M at the enzymatic browning reaction products showed mutagenic action. And also browning solution of 0.05M hydroxyhydroquinone and catechol with Cu^(2+), hydroquinone with and without Cu^(2+) of 0.1M at the enzymatic browning reaction products were strong mutagenic action. The DNA breakability of the enzymatic browning reaction products of 0.1M pyrogallol was stronger than that of 0.05M pyrogallol browning solution with Cu^(2+) and 3,4-dihydroxytoluene browning solution was stronger than that of 0.01M 3,4-dihydroxytoluene browning solution.
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